|
KMID : 0382619840040020505
|
|
Hanyang Journal of Medicine
1984 Volume.4 No. 2 p.505 ~ p.520
|
|
|
A Study of Serum Ribonucleases in Patients with Ovarian Cancer
|
|
|
|
|
Abstract
|
|
|
In the present study activities of neutral and acid ribonucleases (RNases) were determined in serum of patients with serous and mucinous cystadenocarcinoma to find out whether activities of RNases in serum of epithelial ovarian cancer were changed. Neutral RNases in serum of patients with serous cystadenocarcinoma were isolated by ion exchange chromatography and their chromatographic patterns were compared with those of normal persons.
1) While activities of serum acid RNase were not elevated, activities of serum neutral RNase were. greatly increased in both serous and mucinous cystadenocarcinoma.
2) Activities of neutral RNase in serum of the epithelial ovarian cancer were decreased following surgery for removal of the ovarian tumor tissues, increased thereafter to a considerably higher level and maintained the level throughout the postoperative period. Activities of serum neutral RNase in the epithelial ovarian cancer were not influenced by chemotherapeutic measures (against cancer) carried out after the surgery.
3) The ratio of poly C/RNA and poly C/poly TJ for neutral RNase and the ratio of poly C/RNA for acid RNase in serum of epithelial ovarian cancer were unchanged as compared with those of normal. The ratio of poly C/poly U for acid RNase was, however, lower in serum of the ovarian cancer than in normal serum.
4) Neutral RNases in both normal serum and serum of the epithelial ovarian cancer were separated into two peaks by DEAE-cellulose chromatography. The ratio Df p6ak I/peak II for neutral RNases was lesser in the serum of the ovarian cancer than in. . rmal serum.
5) Serum acid RNases were separated into two peaks in normal and ´D three peaks in the epithelial ovarian cancer by DEAE-cellulose chromatography. The substrate specificity for the third peak RNase found in serum of the ovarian cancer appeared to be different from that in normal serum indicating that the third acid RNase might be specific for the cancer.
The observations that neutral RNase activities were greatly increased and that the third acid RNase specific for the cancer was found in serum of the epithelial ovarian cancer might be not only useful as a marker for the cancer but also helpful for understanding development and maintenance of the epithelial ovarian cancer.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|